期刊
NEUROPSYCHOPHARMACOLOGY
卷 46, 期 13, 页码 2340-2349出版社
SPRINGERNATURE
DOI: 10.1038/s41386-021-01146-8
关键词
-
资金
- National Institute on Drug Abuse [R01 DA040630]
This study elucidates the KOR-mediated inhibition of excitatory transmission onto PV-FSIs in the NAc, primarily through promoting AMPAR endocytosis to induce long-term depression. The regulation of PV-FSI synapses by KOR is input specific, and following acute stress, the inhibitory effect of KOR agonists is attenuated.
The dynorphin/kappa opioid receptor (KOR) system within the nucleus accumbens (NAc) contributes to affective states. Parvalbumin fast-spiking interneurons (PV-FSIs), a key component of feedforward inhibition, participate in integration of excitatory inputs to the NAc by robustly inhibiting select populations of medium spiny output neurons, therefore greatly influencing NAc dependent behavior. How the dynorphin/KOR system regulates feedforward inhibition in the NAc remains unknown. Here, we elucidate the molecular mechanisms of KOR inhibition of excitatory transmission onto NAc PV-FSIs using a combination of whole-cell patch-clamp electrophysiology, optogenetics, pharmacology, and a parvalbumin reporter mouse. We find that postsynaptic KOR stimulation induces long-term depression (LTD) of excitatory synapses onto PV-FSI by stimulating the endocytosis of AMPARs via a PKA and calcineurin-dependent mechanism. Furthermore, KOR regulation of PV-FSI synapses are input specific, inhibiting thalamic but not cortical inputs. Finally, following acute stress, a protocol known to elevate dynorphin/KOR signaling in the NAc, KOR agonists no longer inhibit excitatory transmission onto PV-FSI. In conclusion, we delineate pathway-specific mechanisms mediating KOR control of feedforward inhibitory circuits in the NAc and provide evidence for the recruitment of this system in response to stress.
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