期刊
ACTA TROPICA
卷 148, 期 -, 页码 137-141出版社
ELSEVIER
DOI: 10.1016/j.actatropica.2015.04.026
关键词
Permethrin resistance; Detoxification enzymes; Aedes aegypti; Target site insensitivity; Synergist
资金
- DST-SERB Young Scientist Program, New Delhi, India [SR/FT/LS-23/2011]
- Biotechnology Department, Periyar University, Tamil Nadu, India
Pesticide resistance poses a serious problem for worldwide mosquito control programs. Resistance to insecticides can be caused by an increased metabolic detoxification of the insecticide and/or by target site insensitivity. In the present study, we estimated the tolerance of Indian Aedes aegypti populations using adult bioassays that revealed high resistance levels of the field populations to permethrin (RR-6, 5.8 and 5.1 folds) compared to our susceptible population. Enzymatic assays revealed increased activities of glutathione S-transferase and carboxylesterase enzymes in the field populations comparatively to the susceptible population. PBO synergist assays did not confirm that cytochrome P450 monooxygenase metabolic detoxification acted as a major cause of resistance. Hence the role of target site resistance was therefore investigated. A single substitution Phe1534Cys in the voltage gated sodium channel was found in domain III, segment 6 (III-S6) of the resistance populations (allele frequency=0.59, 0.51 and 0.47) suggesting its potential role in permethrin resistance in A. aegypti. (C) 2015 Elsevier B.V. All rights reserved.
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