期刊
FUNGAL BIOLOGY
卷 120, 期 2, 页码 191-206出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2015.08.019
关键词
ITS; mtLSU; Phylogeny; Taxonomy
类别
资金
- EU grant [FP7-261504]
- Autonomous Province of Trento under the project ROCOALPS [PCOFUND-GA-2008-226070]
Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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