Inferior collicular cells that project to the auditory thalamus are increasingly surrounded by perineuronal nets with age

The age-related loss of GABA in the inferior colliculus (IC) likely plays a role in the development of age-related hearing loss. Perineuronal nets (PNs), specialized aggregates of extracellular matrix, increase with age in the IC. PNs, associated with GABAergic neurotransmission, can stabilize synapses and inhibit structural plasticity. We sought to determine whether PN expression increased on GABAergic and nonGABAergic IC cells that project to the medial geniculate body (MG). We used retrograde tract-tracing in combination with immunohistochemistry for glutamic acid decarboxylase and Wisteria floribunda agglutinin across three age groups of Fischer Brown Norway rats. Results demonstrate that PNs increase with age on lemniscal and non-lemniscal IC-MG cells, however two key differences exist. First, PNs increased on non-lemniscal IC-MG cells during middle-age, but not until old age on lemniscal IC-MG cells. Second, increases of PNs on lemniscal IC-MG cells occurred on non-GABAergic cells rather than on GABAergic cells. These results suggest that synaptic stabilization and reduced plasticity likely occur at different ages on a subset of the IC-MG pathway. (c) 2021 Elsevier Inc. All rights reserved.

Automated summary

The age-related loss of GABA and the increase of perineuronal nets in the inferior colliculus may contribute to age-related hearing loss. Specifically, perineuronal nets increase on non-lemniscal IC-MG cells during middle-age, and on lemniscal IC-MG cells only in old age. Additionally, the increase of perineuronal nets on lemniscal IC-MG cells occurs primarily on non-GABAergic cells.