期刊
NATURE PROTOCOLS
卷 16, 期 7, 页码 3241-+出版社
NATURE RESEARCH
DOI: 10.1038/s41596-021-00527-z
关键词
-
资金
- Swiss National Science foundation [P2ZHP3_161770, P400P2 186759]
- Deutsche Forschungsgemeinschaft (German Research Foundation) [DFG - 368482240/GRK2416]
- NIH [5U19NS104649, R01EY022979, 2T32NS064929-11, 5U01NS103489, 5U19NS107613, 1UF1NS107696, 1UF1NS108213, 1RF1MH120680]
- BRAIN initiative [5R01EB026949]
- Army Research Office [W911NF-16-1-0368]
- US DOD
- UK MOD
- UK Engineering and Physical Research Council
- NINDS BRAIN Initiative of the National Institutes of Health [F32MH120888]
- Simons [542963]
- McKnight Foundation
- IARPA MICRONS [D16PC00003]
- DARPA NESD [N66001-17-C-4002]
- Simons Foundation [543023]
- NSF Neuronex Award [DBI-1707398]
- Swiss National Science Foundation (SNF) [P400P2_186759, P2ZHP3_161770] Funding Source: Swiss National Science Foundation (SNF)
Measurements of neuronal activity across different brain areas are crucial for understanding the neural correlates of cognitive and motor processes. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective, and flexible approach that allows high temporal resolution measurements of specific cell populations and a cortex-wide field of view.
Measurements of neuronal activity across brain areas are important for understanding the neural correlates of cognitive and motor processes such as attention, decision-making and action selection. However, techniques that allow cellular resolution measurements are expensive and require a high degree of technical expertise, which limits their broad use. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective and flexible approach to measure activity of specific cell populations with high temporal resolution and a cortex-wide field of view. Here we outline our protocol for assembling a wide-field macroscope setup, performing surgery to prepare the intact skull and imaging neural activity chronically in behaving, transgenic mice. Further, we highlight a processing pipeline that leverages novel, cloud-based methods to analyze large-scale imaging datasets. The protocol targets laboratories that are seeking to build macroscopes, optimize surgical procedures for long-term chronic imaging and/or analyze cortex-wide neuronal recordings. The entire protocol, including steps for assembly and calibration of the macroscope, surgical preparation, imaging and data analysis, requires a total of 8 h. It is designed to be accessible to laboratories with limited expertise in imaging methods or interest in high-throughput imaging during behavior. This protocol describes how to perform long-term wide-field imaging of neuronal activity in behaving mice. The procedure discusses how to assemble and calibrate the macroscope, surgical preparation, imaging and data analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据