期刊
NATURE METHODS
卷 18, 期 6, 页码 604-617出版社
NATURE RESEARCH
DOI: 10.1038/s41592-021-01143-1
关键词
-
资金
- Swiss National Science Foundation [P400PB 180889, 200021-169304]
- NIH [R35 GM122480, R01 DK110520, 1DP2OD007292, 1R01EB018659, 1DP1GM133052, UH3 CA246635, HG009186]
- Welch Foundation [F1515, F-0046]
- Army Research Office [W911NF-12-1-0390]
- Erisyon
- NIGMS [R01 GM138931, P41 GM108569]
- Molecular Robotics Initiative fund at the Wyss Institute for Biologically Inspired Engineering
- Harvard Medical School
- Wyss Institute for Biologically Inspired Engineering
- ERC Advanced Grant Looping DNA [883684]
- NWO
- Azrieli fellowship foundation
- Paul G. Allen Frontiers Program [11715]
- NSF [PHY-1430124]
- NWO-I (SMPS)
- HFSP [RGP0026/2019]
- Polish National Agency for Academic Exchange [PPN/BEK/2018/1/00296]
- European Research Council (ERC 'Enlightened') [616251]
- CEA Transverse Program 'Instrumentation and Detection' (PTC-ID VIRIONEMS)
- Proteomics French Infrastructure (PROFI)
- FNR [C17/BM/11642138]
- Adolphe Merkle Foundation
- Michael J. Fox Foundation for Parkinson's Research [17924]
- European Union's Horizon 2020 research and innovation programme [833399-ERC NanoProt-ID]
- ISF [3485/19]
- I-Site Lille
- Region Hauts-de-France
- European Union's Horizon 2020 Marie Skodowska-Curie [843052]
- project 'International Centre for Cancer Vaccine Science' within the International Agendas Programme of the Foundation for Polish Science - European Union under the European Regional Development Fund
- Genome Canada [264PRO]
- Genome British Columbia [264PRO]
- European Research Council (ERC) [616251, 883684] Funding Source: European Research Council (ERC)
- Marie Curie Actions (MSCA) [843052] Funding Source: Marie Curie Actions (MSCA)
- Swiss National Science Foundation (SNF) [P400PB_180889] Funding Source: Swiss National Science Foundation (SNF)
Single-cell profiling methods have greatly impacted the understanding of cellular heterogeneity, although proteome profiling at the single-cell level is still in the early stages. The development of new technologies, such as single-molecule protein sequencing and innovations in mass spectrometry, will eventually enable broad sequence coverage in single-cell proteomics, leading to new biological discoveries and opportunities for ultra-sensitive disease diagnostics.
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics. This Perspective describes new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell proteomics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据