期刊
NATURE CELL BIOLOGY
卷 23, 期 6, 页码 642-+出版社
NATURE RESEARCH
DOI: 10.1038/s41556-021-00691-0
关键词
-
类别
资金
- National Natural Science Foundation of China [21775115, 32071305, 31670760, 11704286, 11674403]
- Wuhan University
- Fundamental Research Funds for the Central Universities [2042018kf02]
Researchers developed a reversible DNA-based tension probe for quantifying weak forces between cells, which will help to better understand the molecular mechanisms of mechanobiology.
Li et al. develop reversible shearing DNA-based tension probes to quantify molecular piconewton-scale forces, estimate the number of mechanically active receptors with single-molecule sensitivity and study mechanisms of force transduction in live cells. In the last decade, DNA-based tension sensors have made significant contributions to the study of the importance of mechanical forces in many biological systems. Albeit successful, one shortcoming of these techniques is their inability to reversibly measure receptor forces in a higher regime (that is, >20 pN), which limits our understanding of the molecular details of mechanochemical transduction in living cells. Here, we developed a reversible shearing DNA-based tension probe (RSDTP) for probing molecular piconewton-scale forces between 4 and 60 pN transmitted by cells. Using these probes, we can easily distinguish the differences in force-bearing integrins without perturbing adhesion biology and reveal that a strong force-bearing integrin cluster can serve as a 'mechanical pivot' to maintain focal adhesion architecture and facilitate its maturation. The benefits of the RSDTP include a high dynamic range, reversibility and single-molecule sensitivity, all of which will facilitate a better understanding of the molecular mechanisms of mechanobiology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据