期刊
FUNCTIONAL PLANT BIOLOGY
卷 43, 期 9, 页码 838-850出版社
CSIRO PUBLISHING
DOI: 10.1071/FP16064
关键词
antioxidant; ascorbate-glutathione cycle; epigenetics; oxidative stress; recovery; water stress
资金
- FEDER through COMPETE (Programa Operacional Fatores de Competitividade)
- National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-FOR/3807/2012 - FCOMP-01-0124-FEDER-027979, UID/AMB/50017/2013, PTDC/AGR-CFL/112996/2009]
- FCT/MEC
- FEDER [UID/AMB/50017]
- FCT [SFRH/BD/86448/2012, SFRH/BDP/89765/2012, SFRH/BPD/101669/2014]
- Ramon y Cajal Program (Spanish Ministry of Economy and Competitiveness) [RYC-2014-14981]
- Rural and Environment Science and Analytical Services Division of the Scottish Government
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-CFL/112996/2009] Funding Source: FCT
Eucalyptus globulus Labill. is widely cultivated and used by industry but its productivity is currently restricted by drought events, so research focussing on supporting programs to breed adapted germplasm is needed. In the present work we monitored severity of acute drought stress (7 and 11 days after water withholding) and relief (2h and 3 days after rewatering) by quantifying several biochemical markers of oxidative stress and DNA methylation patterns in leaves. Water withholding imposed a mild oxidative stress as estimated by redox shifts in the major antioxidant pools and increased lipid peroxidation. At the DNA level, global 5-methylcytosine distribution increased over the dehydration period especially in vascular tissue as estimated by immunolocalisation. Using methylation-sensitive RAPD analysis, which discriminates methylation changes occurring in specific DNA sequences, we found a high number of specific demethylation events also taking place. Immunolocalisation indicated a rapid reduction in global DNA methylation 2h after rehydration; however, a large number of de novo methylation events were still detected by methylation-sensitive RAPD. These events were associated with decreased lipid peroxidation and high cellular GSH pools relative to unstressed plants. Our results indicate the parallel induction of redox and complex DNA methylation changes occurring during stress imposition and relief.
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