期刊
NANOTECHNOLOGY
卷 32, 期 44, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1361-6528/ac1754
关键词
Au-Ag hollow nanoparticles; surface-enhanced Raman scattering; lateral flow assay; aptamer; prostate cancer
资金
- National Natural Science Foundation of China [81701825]
- Social Development Foundation of Jiangsu [BE2018684]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [17KJB416012]
An innovative biosensor combining surface-enhanced Raman spectroscopy and lateral flow assay with aptamer recognition was developed for convenient, rapid, sensitive and accurate detection of thrombin and PDGF-BB associated with prostate cancer simultaneously. The biosensor operation involves the use of specific aptamers to recognize and capture thrombin and PDGF-BB, allowing for their simultaneous detection in a sandwich structure.
An innovative surface-enhanced Raman spectroscopy and lateral flow assay (SERS-LFA) biosensor combined with aptamer recognition had been developed for the convenient, rapid, sensitive and accurate detection of thrombin and platelet-derived growth factor-BB (PDGF-BB) associated with prostate cancer simultaneously. During the biosensor operation, thrombin and PDGF-BB in the sample were recognized and combined by thiol-modified aptamers immobilized on Au-Ag hollow nanoparticles (Au-Ag HNPs) surface and biotinylated aptamers immobilized on the test lines of the biosensor. Thus, thrombin and PDGF-BB were simultaneously captured between detection aptamers and capture aptamers in a sandwich structure. Finite difference time domain simulation confirmed that 'hot spots' appeared at the gaps of Au-Ag HNPs dimer in the enhanced electromagnetic field compared to that of a single Au-Ag HNP, indicating that the aggregated Au-Ag HNPs owned a good SERS signal amplification effect. The detection limits of thrombin and PDGF-BB in human plasma were as low as 4.837 pg ml(-1) and 3.802 pg ml(-1), respectively. Moreover, the accuracy of the biosensor which was applied to detect thrombin and PDGF-BB in prostate cancer plasma had been verified. This designed biosensor had broad application prospects in the clinical diagnosis of prostate cancer.
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