期刊
NANO TODAY
卷 38, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.nantod.2021.101203
关键词
DNA tetrahedron; Thermophoresis; Extracellular vesicles; MRNA; Detection
资金
- National Natural Science Foundation of China [22025402, 91959101, 21904028]
- Beijing Talents Fund [2018000021223ZK44]
- Chinese Academy of Sciences [YJKYYQ20180055, YJKYYQ20190068, ZDBS-LY-SLH025]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB36000000]
The study developed an ultrasensitive detection method for measuring mRNA in extracellular vesicles, opening up new possibilities for non-invasive and rapid cancer diagnosis. The DNA tetrahedron-based thermophoretic assay achieves a very low limit of detection, allowing for the detection of PSA mRNA in serum without the need for RNA extraction and enzyme amplification, and outperforms serum PSA protein in discriminating between prostate cancer and benign prostatic hyperplasia.
The analysis of messenger RNA (mRNA) in extracellular vesicles (EVs) may provide unprecedented opportunities for non-invasive and rapid diagnosis of cancers. However, clinical utility of EV mRNA is often hampered by the extremely low abundance of mRNA in EVs and the complicated procedures for mRNA detection. Here we address these challenges by developing an ultrasensitive assay for in situ measurement of EV mRNA, which combines FRET-based DNA tetrahedron (FDT) for efficient EV internalization and target mRNA detection, and size-selective thermophoretic accumulation for amplification of FRET signal in EVs. This DNA tetrahedron-based thermophoretic assay (DTTA) achieves a limit of detection (LoD) of 14 aM for detecting PSA mRNA in serum EVs, with resorting to RNA extraction and enzyme amplification. The DTTA showed that EV PSA mRNA outperforms serum PSA protein, the gold standard in PCa screening, for discrimination between prostate cancer and benign prostatic hyperplasia (area under the curve: 0.93 versus 0.74; 42 patients). This technology may greatly expand the applications of DNA nanostructure-enabled liquid biopsy. (c) 2021 Elsevier Ltd. All rights reserved.
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