4.8 Article

Three-Dimensional Quantitative Intracellular Visualization of Graphene Oxide Nanoparticles by Tomographic Flow Cytometry

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Rolling angle recovery of flowing cells in holographic tomography exploiting the phase similarity

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Summary: Holographic tomography allows for 3D mapping of the refractive index of biological samples by reconstructing based on illumination directions or rotation angles, as demonstrated in phase contrast tomographic flow cytometry by digital holography. This method proposes a technique to retrieve rolling angles of cells flowing along microfluidic channels, tested experimentally and validated through numerical simulations, with demonstration achieved in breast cancer cells tomography.

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