期刊
NANO LETTERS
卷 21, 期 14, 页码 5958-5966出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c00868
关键词
nanographene oxide; cellular uptake; tomographic flow cytometry; 3D intracellular spatial distribution of nanoparticles; single cell 3D microscopy
类别
资金
- PRIN [2017N7R2CJ]
This study demonstrates a new method for achieving three-dimensional imaging of nanoparticle distribution in flow cytometry, providing new quantitative descriptions for studying NP-cell interactions.
Interaction of nanoparticles (NPs) with cells is of fundamental importance in biology and biomedical sciences. NPs can be taken up by cells, thus interacting with their intracellular elements, modifying the life cycle pathways, and possibly inducing death. Therefore, there is a great interest in understanding and visualizing the process of cellular uptake itself or even secondary effects, for example, toxicity. Nowadays, no method is reported yet in which 3D imaging of NPs distribution can be achieved for suspended cells in flow-cytometry. Here we show that, by means of label-free tomographic flow-cytometry, it is possible to obtain full 3D quantitative spatial distribution of nanographene oxide (nGO) inside each single flowing cell. This can allow the setting of a class of biomarkers that characterize the 3D spatial intracellular deployment of nGO or other NPs clusters, thus opening the route for quantitative descriptions to discover new insights in the realm of NP-cell interactions.
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