期刊
NANO LETTERS
卷 21, 期 11, 页码 4530-4538出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c05056
关键词
proteomics; PEGylated nanoparticles; protein corona; nanoparticles size analysis
类别
资金
- Fonds de Recherche du Quebec - Nature et Technologies
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Canada Foundation for Innovation (CFI)
- Fonds d'Enseignement et de Recherche of the Faculty of Pharmacy of Universite Laval
- Faculty of Graduate Studies of Universite Laval
This study presents an immunoprecipitation method using AntiPEG antibodies to extract different types of PEGylated systems, showcasing its potential and unique perspective in understanding the interactions of nanoparticles with biological systems.
Polyethylene glycol (PEG) is considered the gold standard to prepare long circulating nanoparticles. The hydrophilic layer that sterically protects PEGylated nanomedicines also impedes their separation from biological media. In this study, we describe an immunoprecipitation method using AntiPEG antibodies cross-linked to magnetic beads to extract three types of radiolabeled PEGylated systems: polymeric nanoparticles, liposomes, and therapeutic proteins. The potential of the method is emphasized by isolating these systems after in vivo administration and ex vivo incubation in human biological fluids. Immunoprecipitation also allows a unique perspective on the size distribution of nanoparticles in the bloodstream after intravenous and intraperitoneal administrations. Further, we highlight the potential of the approach to inform on nanomaterial-associated drug in plasma as well as help characterize the protein corona. Altogether, we believe this method answers an unmet need in nanomedicine research and will contribute a fresh perspective on the interactions of nanomedicines with biological systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据