ATH434 Reduces α-Synuclein-Related Neurodegeneration in a Murine Model of Multiple System Atrophy

Background Multiple system atrophy (MSA) is a fatal neurodegenerative disorder characterized by aggregated alpha-synuclein (alpha-syn) in oligodendrocytes and accompanied by striatonigral and olivopontocerebellar degeneration and motor symptoms. Key features of MSA are replicated in the PLP-alpha-syn transgenic mouse, including progressive striatonigral degeneration and motor deterioration. There are currently no approved treatments for MSA. ATH434 is a novel, orally bioavailable brain penetrant small molecule inhibitor of alpha-syn aggregation. Objectives To characterize ATH434 for disease modification in a mouse model of MSA. Methods Six-month-old PLP-alpha-syn mice (MSA mice) were ATH434-treated (ATH434 in food) or untreated (normal food) for 6 months. Motor behavior and numbers of nigral and striatal neurons were evaluated. alpha-syn aggregates and oligomers were quantified by immunohistochemical and western blot analyses. Microglial activation and neuroinflammation were assessed by histological and molecular analyses. Ferric iron in the Substantia nigra was evaluated with the Perls method. Results ATH434-treated mice demonstrated preservation of motor performance in MSA mice that was associated with neuroprotection of nigral and striatal neurons. The rescue of the phenotype correlated with the reduction of alpha-syn inclusions and oligomers in animals receiving ATH434. ATH434-treated mice exhibited significantly increased lysosomal activity of microglia without increased pro-inflammatory markers, suggesting a role in alpha-syn clearing. ATH434-treatment was associated with lower intracellular nigral iron levels. Conclusions Our findings demonstrate the beneficial disease-modifying effect of ATH434 in oligodendroglial alpha-synucleinopathy on both the motor phenotype and neurodegenerative pathology in the PLP-alpha-syn transgenic mouse and support the development of ATH434 for MSA. (C) 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society

Automated summary

The study demonstrated the disease-modifying effect of ATH434 in a mouse model of MSA, showing neuroprotection of nigral and striatal neurons, reduction of alpha-syn aggregates, and enhanced lysosomal activity of microglia without inducing inflammation.