期刊
MOLECULES
卷 26, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/molecules26175219
关键词
davidone C; Sophora davidii (Franch; ) skeels; anticancer; hepatocellular carcinoma; apoptosis; autophagy
资金
- Special Foundation for International Cooperation in Science and Technology of Qinghai Key Research, Development and Transformation Program [2020-HZ-802]
- National Research Foundation of Korea [NRF-2019K2A9A2A06024397]
- National Natural Science Foundation of China grant [81911540487]
The study found that davidone C inhibited the proliferation of hepatocellular carcinoma cells and induced apoptosis through multiple pathways, possibly involving the mitochondrial apoptotic pathway, ERS pathway, and autophagy signal pathway.
Davidone C is a newly discovered flavonoid compound purified from the ethyl acetate-soluble fraction of Sophora davidii (Franch.) Skeels. This study explored the anti-tumor activity of davidone C on hepatocellular carcinoma HepG2 and Bel-7402 cells and its mechanism through MTT method, morphological observation, flow cytometry and Western blotting. The results showed that davidone C significantly inhibited the proliferation of HepG2 and Bel-7402 cells in a time- and dose-dependent manner. The morphological changes of apoptotic cells can be observed under an inverted microscope, such as cell floating, chromosome condensation, apoptotic bodies, and other phenomena. The expressions of Bax, cleaved caspase-9, cleaved caspase-3 and cleaved PARP increased with the increase of dosage while Bcl-2 decreased, suggesting that the apoptotic mechanism might be related to the mitochondrial apoptotic pathway. Moreover, davidone C administration can down-regulate the expression of Grp78, and simultaneously up-regulate the expression of caspase-7 and caspase-12, indicating that the apoptotic mechanism might be related to the ERS pathway. In addition, davidone C can down-regulate the expression of p62, and simultaneously up-regulate the expression of LC3-I and LC3-II with a quantitative dependence, suggesting that the mechanism of apoptosis may be related to the autophagy signal pathway. All these results showed davidone C has potential effects on hepatocellular carcinoma.
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