期刊
MOLECULES
卷 26, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/molecules26133810
关键词
nerve agent; Novichok; LC-MS; MS; chemical warfare agents
资金
- Agency for Defense Development [912709201]
Nonapeptide adducts containing the active site serine inhibited by ethyl or methyl phosphoramidofluoridate were studied using LC-MS/HRMS, revealing expected biomarkers with a higher abundance of doubly charged ions for trace analysis.
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H](2+) ions than [M+H](+) ions, making doubly charged ions more suitable for trace analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据