期刊
MOLECULES
卷 26, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/molecules26123626
关键词
microbubble; ultrasound; inner ear; drug delivery; insulin-like growth factor-1; noise-induced hearing loss
资金
- Ministry of Science and Technology, Taiwan [MOST107-2314-B-016-027, MOST108-2314-B-016-038, MOST109-2314-B-016-005, MOST107-2314-B-663-001-MY3]
- Medical Affairs Bureau, Ministry of National Defense [MND-MAB-110-061]
- Tri-Service General Hospital [TSGH-A-110001]
The use of ultrasound microbubbles (USMBs) to enhance insulin-like growth factor 1 (IGF-1) delivery through the round window membrane (RWM) shows promising results in treating noise-induced hearing loss (NIHL). The combination of USMBs and IGF-1 leads to improved therapeutic outcomes in inner ear diseases, with increased expression of Akt1 and Mapk3 genes and enhanced immunostaining for phosphor-AKT and phospho-ERK1/2 in the cochlea.
The application of insulin-like growth factor 1 (IGF-1) to the round window membrane (RWM) is an emerging treatment for inner ear diseases. RWM permeability is the key factor for efficient IGF-1 delivery. Ultrasound microbubbles (USMBs) can increase drug permeation through the RWM. In the present study, the enhancing effect of USMBs on the efficacy of IGF-1 application and the treatment effect of USMB-mediated IGF-1 delivery for noise-induced hearing loss (NIHL) were investigated. Forty-seven guinea pigs were assigned to three groups: the USM group, which received local application of recombinant human IGF-1 (rhIGF-1, 10 mu g/mu L) following application of USMBs to the RWM; the RWS group, which received IGF-1 application alone; and the saline-treated group. The perilymphatic concentration of rhIGF-1 in the USM group was 1.95- and 1.67- fold of that in the RWS group, 2 and 24 h after treatment, respectively. After 5 h of 118 dB SPL noise exposure, the USM group had the lowest threshold shift in auditory brainstem response, least loss of cochlear outer hair cells, and least reduction in the number of synaptic ribbons on postexposure day 28 among the three groups. The combination of USMB and IGF-1 led to a better therapeutic response to NIHL. Two hours after treatment, the USM group had significantly higher levels of Akt1 and Mapk3 gene expression than the other two groups. The most intense immunostaining for phosphor-AKT and phospho-ERK1/2 was detected in the cochlea in the USM group. These results suggested that USMB can be applied to enhance the efficacy of IGF-1 therapy in the treatment of inner ear diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据