期刊
MOLECULES
卷 26, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/molecules26154505
关键词
Sanguisorba officinalis L; phenolic acids; triterpenoids; HPLC-ELSD
资金
- Scientific Research Project of National Natural Science Foundation of China [81573551, 81973552]
- Chunhui Program Cooperative Research Project of Ministry of Education of China [HLJ2019018]
A novel analytical method utilizing HPLC-ELSD was developed for simultaneous determination of phenolic acids and triterpenes in Sanguisorba officinalis L. The method was validated to be accurate and effective, allowing for the determination of 23 functional components in Sanguisorba officinalis L. This method could also be applied to study the influence of processing methods on these functional components.
A novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for simultaneous determination of 11 phenolic acids and 12 triterpenes in Sanguisorba officinalis L. Chromatographic separation was conducted with gradient elution mode by using a Diamonsil(TM) C-18 column (250 mm x 4.6 mm, 5 mu m) with the mobile phase of 0.1% acetic acid water (A) and methanol (B). The drift tube temperature of ELSD was set at 70 degrees C and the nitrogen cumulative flow rate was 1.6 L/min. The method was fully validated to be linear over a wide concentration range (R-2 >= 0.9991). The precisions (RSD) were less than 3.0% and the recoveries were between 97.7% and 101.4% for all compounds. The results indicated that this method is accurate and effective for the determination of 23 functional components in Sanguisorba officinalis L. and could also be successfully applied to study the influence of processing method on those functional components in Sanguisorba officinalis L.
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