期刊
MOLECULES
卷 26, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/molecules26175184
关键词
Tobacco Mosaic Virus (TMV); nanoscale wetting; multifrequency AFM; force reconstruction; amplitude-modulation AM-AFM
资金
- Diputacion Foral de Guipuzcoa (MCIU-MINECO, Spain)
- Basque government (MCIU-MINECO, Spain) [PI2013-57, MAT2013-4006-R, PID2019-104650GB-C22]
- Maria de Maeztu Units of Excellence program (MCIU-MINECO, Spain) [MDM-2016-0618]
This study used atomic force microscopy to investigate the nanoscale wetting of Tobacco Mosaic virions, revealing a high sensitivity of multifrequency AFM in visualizing condensed water and sub-micrometer droplets at relative humidity levels above 100%.
The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multi-frequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness similar to 1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据