4.6 Article

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

期刊

MOLECULES
卷 26, 期 18, 页码 -

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MDPI
DOI: 10.3390/molecules26185685

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C-mannosylation; glycobiology; myotube; myogenic differentiation

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  1. Mizutani Foundation for Glycoscience [JP18K06137]

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DPY19L3 is essential for myogenic differentiation, and its knockout affects cell differentiation, leading to suppression of MEK/ERK and p70S6K signaling pathways.
DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

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