期刊
MOLECULAR PLANT PATHOLOGY
卷 22, 期 12, 页码 1613-1623出版社
WILEY
DOI: 10.1111/mpp.13132
关键词
AvrPiz-t; Magnaporthe oryzae; OsUBC26; plant immunity; rice; ubiquitin-conjugating enzyme; WRKY45
资金
- National Natural Science Foundation of China [31972251]
- National Key Research and Development Program of China [2016YFD0100600]
OsUBC26 plays a crucial role in rice disease resistance by regulating WRKY45 expression and cooperating with E3 ligases such as APIP6 to counteract the effector protein AvrPiz-t from Magnaporthe oryzae.
The functions of ubiquitin-conjugating enzymes (E2) in plant immunity are not well understood. In this study, OsUBC26, a rice ubiquitin-conjugating enzyme, was characterized in the defence against Magnaporthe oryzae. The expression of OsUBC26 was induced by M. oryzae inoculation and methyl jasmonate treatment. Both RNA interference lines and CRISPR/Cas9 null mutants of OsUBC26 reduced rice resistance to M. oryzae. WRKY45 was down-regulated in OsUBC26 null mutants. In vitro E2 activity assay indicated that OsUBC26 is an active ubiquitin-conjugating enzyme. Yeast two-hybrid assays using OsUBC26 as bait identified the RING-type E3 ligase UCIP2 as an interacting protein. Coimmunoprecipitation assays confirmed the interaction between OsUBC26 and UCIP2. The CRISPR/Cas9 mutants of UCIP2 also showed compromised resistance to M. oryzae. Yeast two-hybrid screening using UCIP2 as bait revealed that APIP6 is a binding partner of UCIP2. Moreover, OsUBC26 working with APIP6 ubiquitinateds AvrPiz-t, an avirulence effector of M. oryzae, and OsUBC26 null mutation impaired the proteasome degradation of AvrPiz-t in rice cells. In summary, OsUBC26 plays important roles in rice disease resistance by regulating WRKY45 expression and working with E3 ligases such as APIP6 to counteract the effector protein AvrPiz-t from M. oryzae.
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