期刊
MOLECULAR PLANT
卷 14, 期 11, 页码 1881-1900出版社
CELL PRESS
DOI: 10.1016/j.molp.2021.07.013
关键词
Soybean mosaic virus; Rsc4; NLR; resistance gene; cell wall; palmitoylation
资金
- National Natural Science Foundation of China [31571690, 31770164]
- Fundamental Research Funds for the Central Universities [KYT201801]
- Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT_17R55]
- National Soybean Industrial Technology System of China [CARS-004]
- Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)
- National Key R&D Program of China [2017YFD0101501]
- Natural Science Foundation of Jiangsu Province [BK20180039]
In this study, a unique NLR that recognizes plant virus in the apoplast was identified, providing a simple and effective method for identifying resistant genes against SMV infection.
Soybean mosaic virus (SMV) causes severe yield losses and seed quality reduction in soybean (Glycine max) production worldwide. Rsc4 from cultivar Dabaima is a dominant genetic locus for SMV resistance, and its mapping interval contains three nucleotide-binding domain leucine-rich repeat-containing (NLR) candidates (Rsc4-1, Rsc4-2, and Rsc4-3). The NLR-type resistant proteins were considered as important intracellular pathogen sensors in the previous studies. In this study, based on transient expression assay in Nicotiana benthamiana leaves, we found that the longest transcript of Rsc4-3 is sufficient to confer resistance to SMV, and CRISPR/Cas9-mediated editing of Rsc4-3 in resistant cultivar Dabaima compromised the resistance. Interestingly, Rsc4-3 encodes a cell-wall-localized NLR-type resistant protein. We found that the internal polypeptide region responsible for apoplastic targeting of Rsc4-3 and the putative palmitoylation sites on the N terminus are essential for the resistance. Furthermore, we showed that viral encoded cylindrical inclusion (CI) protein partially localizes to the cell wall and can interact with Rsc4-3. Virus-driven or transient expression of CI protein of avirulent SMV strains is enough to induce resistance response in the presence of Rsc4-3, suggesting that CI is the avirulent gene for Rsc4-3-mediated resistance. Taken together, our work identified a unique NLR that recognizes plant virus in the apoplast, and provided a simple and effective method for identifying resistant genes against SMV infection.
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