期刊
BRITISH JOURNAL OF CANCER
卷 112, 期 5, 页码 874-882出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/bjc.2015.3
关键词
kinesin light chain-2; miR-125b; non-small-cell lung cancer; prognosis; migration
类别
资金
- National Natural Science Foundation of China [81001047, 81071827]
- Science and technology projects in Guangdong Province [2012B031800127]
- Excellent Young Teachers Program of Higher Education of Guangdong Province [Yq2013040]
- Research Fund for the Science and Technology Star of Zhujiang of Guangzhou City [2014J2200031]
Background: MiR-125b has critical role in non-small-cell lung cancer (NSCLC) cell migration, and its target genes have not been elucidated. Kinesin-1 light chain (KLC)-2 was predicted as one of miR-125b's targets by bioinformatics analysis. This study is to identify the function of KLC2 and its interaction with miR-125b in NSCLC. Methods: Kinesin-1 light chain-2 protein expression and its clinical relevance were analysed in 140 matched NSCLC and adjacent non-neoplastic lung tissues. Both KLC2 gain-and loss-of-function analyses were performed in NSCLC cell lines by transient transfection. The direct interaction between KLC2 and miR-125b was confirmed by a luciferase reporter assay and a transient co-transfection assay as well as an analysis of eight matched clinical samples. Results: KLC2 protein was upregulated in NSCLC cell lines and tissues, and was an independent predictor of poor prognosis for elderly NSCLC patients. Kinesin-1 light chain-2 remarkably enhanced the invasive and migratory ability of NSCLC cells. MiR-125b inhibited KLC2 30-untranslated region luciferase activity and protein expression, and inversely correlated with KLC2 expression in clinical samples. Kinesin-1 light chain-2 almost completely reversed miR-125b-induced inhibition on migration and invasion. Conclusions: Kinesin-1 light chain-2 protein overexpression predicts poor survival in elderly NSCLC patients. Kinesin-1 light chain-2 acts as a proto-oncogene and a functional target of miR-125b in NSCLC cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据