期刊
MOLECULAR ONCOLOGY
卷 15, 期 9, 页码 2466-2479出版社
WILEY
DOI: 10.1002/1878-0261.13029
关键词
breast cancer; circulating exosome; lymph node metastasis; miR-363-5p; miRNA; PDGFB
类别
资金
- National Natural Science Foundation of China [81802669]
- CAMS Initiative Fund for Medical Sciences [2016-I2M-1-001, 2017-I2M-3-004]
- Zhejiang Provincial Natural Science Foundation of China [LY21C060004]
Plasma exosomal miR-363-5p plays a tumor suppressor role in breast cancer and has the potential for noninvasive LN staging and prognosis prediction.
Sentinel lymph node (LN) biopsy is currently the standard procedure for clinical LN-negative breast cancer (BC) patients but it is prone to false-negative results and complications. Thus, an accurate noninvasive approach for LN staging is urgently needed in clinical practice. Here, circulating exosomal microRNA (miRNA) expression profiles in peripheral blood from BC patients and age-matched healthy women were obtained and analyzed. We identified an exosomal miRNA, miR-363-5p, that was significantly downregulated in exosomes from plasma of BC patients with LN metastasis which exhibited a consistent decreasing trend in tissue samples from multiple independent datasets. Plasma exosomal miR-363-5p achieved high diagnostic performance in distinguishing LN-positive patients from LN-negative patients. The high miR-363-5p expression level was significantly correlated with improved overall survival. Functional assays demonstrated that exosomal miR-363-5p modulates platelet-derived growth factor (PDGF) signaling activity by targeting PDGFB to inhibit cell proliferation and migration. Our study revealed, for the first time, plasma exosomal miR-363-5p plays a tumor suppressor role in BC and has the potential for noninvasive LN staging and prognosis prediction of BC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据