期刊
MOLECULAR BIOLOGY REPORTS
卷 48, 期 9, 页码 6471-6480出版社
SPRINGER
DOI: 10.1007/s11033-021-06653-x
关键词
Capoeta aculeata; Microsatellite markers; Next generation sequencing (NGS); Genetic structure
资金
- Research Fund for the department of Fisheries of the Islamic Azad University of Tehran, Iran
This study identified 36 novel polymorphic microsatellite markers and analyzed the population genetic structure of Capoeta aculeata sampled from three geographical sites in Iran using next generation sequencing technology. The results showed potential for these markers to be valuable tools for future population and conservation genetics studies of C. aculeata and related species.
Background The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of the population genetic structure of this species is limited by the low number of molecular markers currently described. Methods and results In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16 (average = 7.89). The results showed that, the polymorphism information content (PIC) of these simple sequence repeat (SSR) loci varied from 0.254 to 0.888. The observed heterozygosity (H-O) per locus ranged from 0.170 to 0.881, while the expected heterozygosity (H-E) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated significantly from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05). Conclusions These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate and other closely related species.
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