期刊
MOLECULAR BIOLOGY REPORTS
卷 48, 期 10, 页码 6923-6934出版社
SPRINGER
DOI: 10.1007/s11033-021-06695-1
关键词
Carotenoids; Neoxanthin; HepG2 cells; Cytoprotection; Antioxidant enzymes; Apoptosis
资金
- Department of Biotechnology (DBT), New Delhi, India
- CSIR-CFTRI
The study demonstrates that neoxanthin provides protection against stress-induced cytotoxicity in hepatocytes by activating the intracellular antioxidant defense system and blocking apoptosis.
Background The liver has a solid inbuilt antioxidant defense system to regulate oxidative stress. However, exposure to an excessive level of ROS causes liver injury. This study examined the cytoprotective effect of neoxanthin, a xanthophyll antioxidant molecule isolated from Solanum trilobatum in stress-induced HepG2 cells. Methods and results The cytotoxic effect of H2O2 and cytoprotective potential of beta-carotene, lutein, and neoxanthin was analyzed by WST-1 assay. The intracellular ROS level and mitochondrial membrane potential (MMP) were measured using DCFH-DA (2 ', 7 '-dichlorofluorescin diacetate) and JC-10 MMP assay. The expression of anti-oxidant and apoptotic markers was measured by western blot analysis. Neoxanthin pretreatment exhibited better protection than beta-carotene and lutein against cell death caused by H2O2. It significantly arrested H2O2-mediated elevation of intracellular ROS levels and protected MMP. The intracellular antioxidant enzymes HO-1 and SOD-2 were upregulated by neoxanthin pretreatment. Neoxanthin also activated the protein expression of redox-sensitive transactivation factors, Nrf2 and NF-kB. The cytoprotective effect of neoxanthin was associated with increased expression of the anti-apoptotic protein, Bcl-2 and decreased pro-apoptotic protein Bax. Conclusions For the first time, our results demonstrate that neoxanthin offers adequate protection against stress-mediated cytotoxicity in hepatocytes by activating the intracellular antioxidant defense system and blocking apoptosis.
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