期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 38, 期 11, 页码 4934-4947出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msab218
关键词
functional characterization; neofunctionalization; odorant receptor; olfaction; purifying selection; Xenopus oocyte
资金
- Chinese Scholarship Council (CSC)
- Swedish Research Councils FORMAS [217-2014-689, 2018-01444, 2017-03804]
- Crafoord foundation
- Carl Trygger Foundation [CTS 17:25]
- Royal Physiographic Society in Lund
- Formas [2018-01444] Funding Source: Formas
- Vinnova [2017-03804] Funding Source: Vinnova
- Swedish Research Council [2017-03804] Funding Source: Swedish Research Council
This study investigated the functional evolution of odorant receptors (ORs) in the Eurasian spruce bark beetle Ips typographus, revealing that these receptors primarily respond to compounds from host trees and beetle-associated fungi. The ORs showed a shared property of responding to monoterpenoids, indicating early neofunctionalization following gene duplication in this lineage.
Insects detect odors using an array of odorant receptors (ORs), which may expand through gene duplication. How and which new functions may evolve among related ORs within a species remain poorly investigated. We addressed this question by functionally characterizing ORs from the Eurasian spruce bark beetle Ips typographus, in which physiological and behavioral responses to pheromones, volatiles from host and nonhost trees, and fungal symbionts are well described. In contrast, knowledge of OR function is restricted to two receptors detecting the pheromone compounds (S)-(-)-ipsenol (ItypOR46) and (R)-(-)-ipsdienol (ItypOR49). These receptors belong to an Ips-specific OR-lineage comprising seven ItypORs. To gain insight into the functional evolution of related ORs, we characterized the five remaining ORs in this Glade using Xenopus oocytes. Two receptors responded primarily to the host tree monoterpenes (+)-3-carene (ItypOR25) and p-cymene (ItypOR27). Two receptors responded to oxygenated monoterpenoids produced in larger relative amounts by the beetle-associated fungi, with ItypOR23 specific for (+)-trans-(1R, 4S)-4-thujanol, and ItypOR29 responding to (+)-isopinocamphone and similar ketones. ItypOR28 responded to the pheromone E-myrcenol from the competitor Ips duplicatus. Overall, the OR responses match well with those of previously characterized olfactory sensory neuron classes except that neurons detecting E-myrcenol have not been identified. The characterized ORs are under strong purifying selection and demonstrate a shared functional property in that they all primarily respond to monoterpenoids. The variation in functional groups among OR ligands and their diverse ecological origins suggest that neofunctionalization has occurred early in the evolution of this OR-lineage following gene duplication.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据