期刊
MICROCHIMICA ACTA
卷 188, 期 8, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-021-04935-6
关键词
Photoelectrochemical biosensor; microRNA; Homogeneous; Target-responsive hydrogel; Signal amplification
资金
- National Key Research and Development Program of China [2019YFC1604701]
- National Sciences Foundation of China [21974020, 21775026]
- cooperative project of production and study in University of Fujian Province [2018Y4007]
- Sciences Foundation of Fujian Province [2018 J01685, 2018J01682]
- United Fujian Provincial Health and Education Project for Tackling the Key Research P. R. China [2019-WJ-11]
A novel homogeneous PEC biosensor based on target-responsive hydrogels has been developed for sensitive detection of miRNA-155. By utilizing a cascade amplification strategy, the biosensor exhibits high sensitivity with a low detection limit of 0.41 fM and a wide linear range from 1.0 fM to 100 pM. The simplicity, reproducibility, and storage stability of the prepared hydrogels make this biosensing platform versatile for diverse target detection.
MicroRNAs can serve as biomarkers for many cancers, so it is significant to develop simple and sensitive strategies for microRNAs detection. Photoelectrochemical (PEC) detection has the advantages of simple equipment and high sensitivity But in conventional PEC DNA sensors, tedious immobilization procedures of photoactive materials and capture probes on electrode surfaces are inevitable. To overcome those limitations, a homogeneous PEC biosensor based on target-responsive hydrogels has been developed (miRNA-155 has been chosen as a model target). PEC signal molecules (TiO2 nanoparticles, TiO2 NPs) were embedded in DNA hydrogels formed by hyaluronic acid sodium salt, amine-modified DNA double strands, and polyethylenimine rich in amine groups. In the presence of the target, DNA double strands in hydrogel were nicked by endonuclease and TiO2 NPs were released to the supernate and a high PEC response was obtained when collecting the supernate for PEC test, while almost no TiO2 NPs released in the absence of the target. Thanks to the exonuclease III and nicking endonuclease Nb.BbvCI-assisted cascaded amplification strategy, the proposed biosensor exhibits high sensitivity toward miRNA-155 with a low detection limit of 0.41 fM and a wide linear range from 1.0 fM to 100 pM. Since this method circumvents tedious electrode modification procedures, the proposed technique exhibits the advantages of simplicity and good reproducibility. Moreover, the prepared hydrogels have outstanding storage stability, so that they can be prepared in advance and shorten detection time. This biosensing platform provides a versatile strategy for the construction of homogeneous PEC biosensors for the detection of diverse targets.
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