4.7 Article

Nanoparticle-based fluorescence probe for detection of NF-κB transcription factor in single cell via steric hindrance

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A CRISPR-derived biosensor for the sensitive detection of transcription factors based on the target-induced inhibition of Cas12a activation

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Summary: Transcription factors (TFs) are crucial proteins for cell fate determination and have potential applications in disease diagnostics and treatment. A highly sensitive biosensor based on the CRISPR/Cas12a system was developed for TF detection, utilizing competitive binding between Cas12a-crRNA and TFs to a specific dsDNA activator. This study demonstrates a novel approach in applying CRISPR technology for TF sensing and highlights the potential of using blocking mechanisms as a new sensing strategy for CRISPR-based biosensors.

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