期刊
MICROCHIMICA ACTA
卷 188, 期 7, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-021-04880-4
关键词
Alkaline phosphatase; Lanthanide metal-organic framework; Nanozyme; Ratiometric fluorescence; Visual detection
资金
- National Natural Science Foundation of China [82073811, 81673394]
- Fundamental Research Funds for the Central Universities [2042020kf1010]
A turn-on/off ratiometric fluorescence detection platform using multifunctional Ln-MOF and an enzymatic cascade reaction has been proposed for alkaline phosphatase (ALP) activity assay. The method showed good linear relationship and low detection limit, with successful application in human serum samples for monitoring ALP activity.
A turn-on/off ratiometric fluorescence detection platform based on multifunctional lanthanide metal-organic framework (Ln-MOF) and an enzymatic cascade reaction is proposed for alkaline phosphatase (ALP) activity assay. L-phosphotyrosine is hydrolyzed to levodopa (L-dopa) by two steps of enzymatic reaction. L-dopa further reacts with naphthoresorcinol to produce carboxyazamonardine with strong emission at 490 nm. In this process, multifunctional Ln-MOF (Cu@Eu-BTC, BTC is the 1,3,5-benzenetricarboxylic acid) acts not only as a nanozyme to catalyze the fluorogenic reaction between L-dopa and naphthoresorcinol but also as a fluorescence internal standard. The emission of Cu@Eu-BTC at 620 nm is quenched by phosphate anions, and the dual-response ratiometric fluorescence (F-490/F-620) can be achieved. A good linear relationship was obtained between Delta(F-490/F-620) and ALP activity in the range 0.3-24 U L-1 with the detection limit of 0.02 U L-1. In addition, a portable assay tube was designed for visual and point-of-care testing of ALP activity by color variation (ratiometric chromaticity). Both the ratiometric fluorescence detection and the visual detection methods were successfully applied to monitor ALP activity in human serum samples with recovery between 95.5%-109.0% and 94.0%-110.1%, and relative standard deviation less than 8.1% and 9.5%, respectively. As far as we know, this is the first report of ALP activity assay assisted by multifunctional Ln-MOF.
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