期刊
MICROCHIMICA ACTA
卷 188, 期 10, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-021-04988-7
关键词
Electrochemiluminescence; Core-shell nanoparticles; Immunosensor; CeO2-Au@Pt; Au star@BSA
资金
- National Natural Science Foundation of China [21777056, 21675063]
- Innovation Team Project of Colleges and Universities in Jinan [2019GXRC027]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- Jinan Scientific Research Leader Workshop Project [2018GXRC021, 2018GXRC024]
A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was successfully fabricated for highly sensitive detection of procalcitonin (PCT), demonstrating great potential for clinical applications.
A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). CeO2-Au@Pt composed of sea urchin-like Au@Pt nanoparticles coated on CeO2 hollow nanospheres was immobilized on electrode surface to electrochemically catalyze H2O2 to produce a large number of superoxide anion (O-2(center dot-)). The immunosensor was prepared by linking the capture antibody on immobilized CeO2-Au@Pt with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au star@BSA was used to bind abundant luminol for labeling the secondary antibody (Ab(2)). Upon the sandwich-typed immunoreactions, the O-2(center dot-) could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low H2O2 concentration and demonstrated its high application potential in the clinical assay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据