期刊
MICROCHIMICA ACTA
卷 188, 期 10, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-021-04995-8
关键词
miRNA multiplex detection; Electrochemical biosensors; Breast cancer; Screen printed carbon electrode; Nanoparticle modified electrode
资金
- National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA) [P1851652]
- National Nanotechnology Center (NANOTEC)
- National Science and Technology Development Agency (NSTDA)
A highly sensitive electrochemical biosensor has been developed for the detection of multiplex micro ribonucleic acids (miRNAs), showing high selectivity, stability, and sensitivity. It is capable of detecting breast cancer biomarkers miRNA-155, miRNA-16, and miRNA-21 simultaneously, making it a potential tool for breast cancer screening in normal human serum.
A highly sensitive electrochemical biosensors has been developed for the detection of multiplex micro ribonucleic acids (miRNAs) by modifying an electrode with reduced graphene oxide/poly(2-aminobenzylamine)/gold nanoparticles and adopting porous, hollow silver-gold nanoparticles as tagged labeling with metal ions. In addition, an anti-deoxyribonucleic acid (DNA)-RNA hybrid [S9.6] antibody was used to detect different hybridized capture DNAs and miRNAs that can detect multiple miRNAs simultaneously. The developed electrochemical platform exhibits high selectivity, stability, and sensitivity with a wide linear range from 1 fM to 10 nM and a low detection limit of 0.98 fM, 3.58 fM, and 0.25 fM for miRNA-155, miRNA-21, and miRNA-16, respectively. In addition, the proposed electrochemical biosensor capable for the simultaneous detection of miRNA-155, miRNA-16, and miRNA-21, which are breast cancer biomarkers, in normal human serum, can be adopted and potentially used for breast cancer screening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据