Combination of ultrathin micro-patterned MXene and PEDOT: Poly(styrenesulfonate) enables organic electrochemical transistor for amperometric determination of survivin protein in children osteosarcoma

An ultrathin micro-patterned MXene/PEDOT:PSS-based organic electrochemical transistor biosensor was constructed, which can significantly amplify the amperometric signal and transistor's performance. A novel interdigitated OECTs biosensor has been developed for reliable determination of survivin for the following considerations: (1) The synergistic effect of intercalated MXene and ionic PEDOT:PSS enhanced the mobility and volumetric capacitance of OECTs biosensor. (2) Compared with the best previous literatures, our assay demonstrated enhanced detection limit of survivin down to 10 pg mL(-1), as well as satisfactory selectivity, reproducibility, and reliability. (3) Comparison of OECTs against commercial ELISA kit yielded favorable linearity (Y = 1.0015*X + 0.0039) and correlation coefficient (R-2 = 0.9717). Those advantages are expected to pave the way to design of an OECTs biosensor with robustness, non-invasiveness, and miniaturization for the point-of-care applications.

Automated summary

In this study, an ultrathin micro-patterned MXene/PEDOT:PSS-based organic electrochemical transistor biosensor was developed to amplify the amperometric signal and improve transistor's performance for the reliable determination of survivin. The novel interdigitated OECTs biosensor exhibited enhanced detectability, selectivity, reproducibility, and reliability compared to previous literatures, with favorable linearity and correlation coefficient when compared against a commercial ELISA kit. These advantages are expected to pave the way for the design of robust, non-invasive, and miniaturized OECTs biosensors for point-of-care applications.