Direct quantification of cysteine and glutathione by 1H NMR based on β-cyclodextrin modified silver nanoparticles

Sulfhydryl-containing amino acids had a variety of biological functions. Herein, a method was simply set up to determine Cys and GSH with beta-CD AgNPs quantitatively. The chemical shifts of Cys and GSH both moved to higher field when beta-CD AgNPs were added. The reason was that the sulfhydryl groups could bind to the surface of AgNPs. In addition, the competitive effect of Cys and GSH in mixed solutions was explored via the 1H chemical shift deviation. Peaks at 3.05 ppm and 2.93 ppm were selected as characteristic signals. This method exhibited an excellent linearity in the range of Cys and GSH in 0.1-20 mM. The detection limits of Cys and GSH were 40 mu M and 14 mu M, respectively. This method was successfully used to quantitatively determine Cys and GSH in human serum and urine.

Automated summary

A method was established to quantitatively determine Cys and GSH by studying their interaction with β-CD AgNPs using chemical shift analysis. The method exhibited good linearity and low detection limits, and was successfully applied for quantifying Cys and GSH in human serum and urine.