Poly amidoamine functionalized poly (styrene-divinylbenzene-glycidylmethacrylate) composites for the rapid enrichment and determination of N-phosphoryl peptides

In this work, poly amidoamine grafted composites were proposed as enrichment adsorption materials. The composites were synthesized by grafting poly amidoamine dendrimers onto the surface of poly (styrene-divinylbenzene-glycidylmethacrylate) beads. The number of surface functional groups and adsorption capacities of the composites were controllable by varying the generations of poly amidoamine dendrimers. Afterward, adsorption parameters and desorption eluents were optimized by using N-phosphoryl dipeptide as a target molecular. Under the optimized condition, N-phosphoryl dipeptides in aqueous could be rapidly adsorbed within 30 s, and comparatively high selectivity, enrichment efficiency and recovery efficiency were obtained at pH 3 to 7. The used adsorbents show good reproducibility after being washed by methanol solvent. Reliability of the adsorbents was demonstrated by the determination of N-phosphoryl dipeptide in drinking water samples with the efficient dispersive solid phase extraction-high performance liquid chromatography method. According to the obtained result, the adsorbent showed satisfactory repeatability (RSD <= 5.51) and good recoveries (90.52-102.84%).

Automated summary

Poly amidoamine grafted composites were proposed as enrichment adsorption materials for the rapid and efficient adsorption of N-phosphoryl dipeptide. Controllability of surface functional groups and adsorption capacities of the composites were achieved by varying the generations of poly amidoamine dendrimers. Under optimized conditions, high selectivity and recovery efficiency were obtained, demonstrating good reproducibility in practical samples.