期刊
MICROCHEMICAL JOURNAL
卷 171, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2021.106846
关键词
Exosomes; Personal glucose meter; Rolling circle amplification; POCT
The proposed method utilizes a commercial personal glucose meter for exosomes quantification, translating signals to achieve sensitive detection of exosomes, with potential application in clinical diagnosis for early detection of atherosclerosis.
Exosomes, function as an important mediator of inter-cellular communication in the release of inflammatory cytokines and over-activation of inflammatory responses, are closely related to the occurrence and development of Atherosclerosis (AS). However, It remains a huge challenge to develop a cost-effective method for early identification of exosomes. Herein, we proposed a simple, portable and sensitive biosensor for exosomes quantification using commercial personal glucose meter (PGM) for signal readout. Highlights of the proposed method can be concluded as: i) the signals of exosomes were translated to signals of nucleic acids through an elegantly designed capture probe, coupling with signal amplification strategies to improve sensitivity; ii) with the assistance of DNA-invertase, the amplified nucleic acids signals are converted into glucose signals that can be detected by PGM; iii) apart from the portability, the proposed method also exhibits an exosomes detection range from 103 to 106 particles/mu l. We believe that the established method can be potentially applied in clinical diagnosis as a point-of-care testing (POCT) tool and thus contribute to the early-diagnosis of atherosclerosis.
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