期刊
MICROBIAL ECOLOGY
卷 83, 期 4, 页码 1008-1025出版社
SPRINGER
DOI: 10.1007/s00248-021-01818-4
关键词
Ensifer fredii NGR234; Root exudates (RE); Cajanus cajan; Zea mays; Metabolomics; Proteomics
资金
- University Grant Commission, New Delhi, India
- Science and Engineering Research Board, Department of Science and Technology, Govt. of India [JCB/2017/000053]
Legume-cereal intercropping systems enhance plant-rhizobia interactions by adjusting the composition of root exudates (RE). The metabolites of RE differ in Cajanus cajan-Zea mays intercropped plants, affecting the biofilm formation and growth rate of rhizobia. Proteomics analysis highlights potential molecular candidates involved in the fitness of rhizobium in the intercropping system.
Legume-cereal intercropping systems, in the context of diversity, ecological function, and better yield have been widely studied. Such systems enhance nutrient phytoavailability by balancing root-rhizosphere interactions. Root exudates (RE) play an important role in the rhizospheric interactions of plant-plant and/or plant-microbiome interaction. However, the influence of the primary metabolites of RE on plant-rhizobia interactions in a legume-cereal intercrop system is not known. To understand the plant communication with rhizobia, Cajanus cajan-Zea mays intercropped plants and the broad host range legume nodulating Ensifer fredii NGR234 as the model plants and rhizobium used respectively. A metabolomics-based approach revealed a clear separation between intercropped and monocropped RE of the two plants. Intercropped C. cajan showed an increase in the myo-inositol, and proline, while intercropped Z. mays showed enhanced galactose, D-glucopyranoside, and arginine in the RE. Physiological assays of NGR234 with the RE of intercropped C. cajan exhibited a significant enhancement in biofilm formation, while intercropped Z. mays RE accelerated the bacterial growth in the late log phase. Further, using label-free proteomics, we identified a total of 2570 proteins of NGR234 covering 50% annotated protein sequences upon exposure to Z. mays RE. Furthermore, intercropped Z. mays RE upregulated bacterioferritin comigratory protein (BCP), putative nitroreductase, IlvD, LeuC, D (branched-chain amino acid proteins), and chaperonin proteins GroEL2. Identification offered new insights into the metabolome of the legume-cereal intercrop and proteome of NGR234-Z. mays interactions that underline the new molecular candidates likely to be involved in the fitness of rhizobium in the intercropping system.
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