期刊
MICROBIAL DRUG RESISTANCE
卷 27, 期 11, 页码 1546-1554出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/mdr.2020.0175
关键词
carbapenemases; NDM-1; OXA-23; VIM-2; OprD; PFGE
资金
- IHU Mediterranee Infection, Aix-Marseille-University in France
- Tunisian Ministry of Higher Education and Scientific Research
- Campus France Under the PHCUtique project [18G0819]
This study investigated the mechanism of carbapenem resistance in A. baumannii and P. aeruginosa isolates from a Libyan hospital, identifying the dissemination of bla(OXA-23) and bla(NDM-1) genes. Infection prevention and control practices should be implemented to prevent the wide spread of antimicrobial resistance.
Acinetobacter baumannii and Pseudomonas aeruginosa are among the most prevalent pathogens causing a wide range of serious infections in hospitalized patients and contaminating intensive care units and inanimate surfaces. The purpose of this study was to investigate the mechanism of carbapenem resistance in clinical and hospital environmental isolates of A. baumannii and P. aeruginosa recovered from a Libyan hospital. From a total of 82 Gram-negative bacteria, 8 isolates of A. baumannii and 3 isolates of P. aeruginosa exhibited resistance to imipenem with minimum inhibitory concentrations ranging from 16 to >32 mu g/mL. Five isolates of A. baumannii harbored bla(OXA-23) gene, from which three isolates were collected from patients and two from hospital environment. Only one isolate harbored bla(NDM-1) gene, which was responsible for carbapenem resistance in A. baumannii. The OprD gene seems to be disturbed by an insertion sequence (IS) in two isolates and affected by polymorphism in one isolate. Pulsed-field gel electrophoresis results showed high genetic diversity among carbapenemase producing A. baumannii. This study highlights the dissemination of bla(OXA-23) and bla(NDM-1) genes in a Libyan setting. Therefore, infection prevention and control practices, antimicrobial stewardship initiatives, and antimicrobial resistance surveillance systems should be implemented to prevent the wide spread of antimicrobial resistance.
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