期刊
METABOLIC ENGINEERING
卷 67, 期 -, 页码 53-66出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2021.05.010
关键词
Outer membrane; Halomonas; Polyhydroxyalkanoates; PHB; Lipopolysaccharide; Permeability; Next generation industrial biotechnology
资金
- Ministry of Science and Technology of China [2018YFA0900200]
- National Natural Science Foundation of China [31961133019, 21761132013, 31870859, 31961133017, 31961133018]
- Center of Life Sciences of Tsinghua-Peking University
- European Union's Horizon 2020 research and innovation programme [870294]
The bacterial outer membrane (OM) plays a crucial role in bioproduction, with OM-defected Halomonas bluephagenesis strains showing improved adaptation to lower salinity and increased production of bioproducts. The study highlights the significant impact of OM defection on metabolic engineering, synthetic biology studies, and industrial applications.
Bacterial outer membrane (OM), an asymmetric lipid bilayer functioning as a self-protective barrier with reduced permeability for Gram-negative bacteria, yet wasting nutrients and energy to synthesize, has not been studied for its effect on bioproduction. Here we construct several OM-defected halophile Halomonas bluephagenesis strains to investigate the effects of OM on bioproduction. We achieve enhanced chassis properties of H. bluephagenesis based on positive cellular properties among several OM-defected strains. The OM-defected H. bluephagenesis WZY09 demonstrates better adaptation to lower salinity, increasing 28%, 30% and 12% on dry cell mass (DCM), poly(3-hydroxybutyrate) (PHB) accumulation and glucose to PHB conversion rate, respectively, including enlarged cell sizes and 21-folds reduced endotoxin. Interestingly, a poly(3-hydroxybutyrate-co-21mol%4hydroxybutyrate) (P(3HB-co-21mol%4HB)) is produced by H. bluephagenesis WZY09 derivate WZY249, increasing 60% and 260% on polyhydroxyalkanoate (PHA) production and 4HB content, respectively. Furthermore, increased electroporation efficiency, more sensitive isopropyl beta-D-1-thio-galactopyranoside (IPTG) induction, better oxygen uptake, enhanced antibiotics sensitivity and ectoine secretion due to better membrane permeability are observed if OM defected, demonstrating significant OM defection impacts for further metabolic engineering, synthetic biology studies and industrial applications.
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