期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2021.112295
关键词
Electrospun scaffold; Layer-by-layer self-assembly; Apatite mineralization; Bone regeneration
资金
- National Natural Science Foundation of China [82072406, 51790501]
- Key Program of BeijingNatural Science Foundation [Z200025]
- Beijing Municipal Health Commission [PXM2020-026275-000003, BMHC2019-9]
- Beijing Nova Programme Interdisciplinary Cooperation Project [Z191100001119012]
- Fundamental Research Funds for the Central Universities
- Louisi-ana Board of Regents, USA [RDD-03]
The development of osteoinductive artificial scaffolds is crucial for bone repair and regeneration. By combining layer-by-layer nanocoating and mineralization, a biomimetic apatite interface was constructed on polycaprolactone fibers, promoting adhesion, proliferation, and osteogenic differentiation of cells, as well as enhancing ectopic osteogenesis in vivo. These nanoarchitectural scaffolds mimic the composition and structure of bone matrix, showing great potential for bone repair and regeneration.
It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration. We constructed a biomimetic apatite interface on electmspun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold. After polydopamine modification, cationic type-1 collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface. The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition. By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized. When ten bilayers of the collagen and chondmitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis. Our nanoarchitectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.
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