期刊
MASS SPECTROMETRY REVIEWS
卷 42, 期 1, 页码 131-143出版社
WILEY
DOI: 10.1002/mas.21715
关键词
cycle time; DDA; exclusion list; mass window; precursor selection; Q-TOF; tandem mass spectrometry
类别
Metabolomics has become a pivotal approach for analyzing metabolites in biological systems, allowing for a deeper understanding of the relationship between biochemical processes and physiological conditions. Targeted and untargeted mass spectrometry methods are commonly used, with data-dependent acquisition (DDA) offering the potential for improved metabolite assignment in untargeted metabolomics. However, DDA settings are more complex and prone to errors compared to data-independent acquisition (DIA).
In recent years, metabolomics has emerged as a pivotal approach for the holistic analysis of metabolites in biological systems. The rapid progress in analytical equipment, coupled to the rise of powerful data processing tools, now provides unprecedented opportunities to deepen our understanding of the relationships between biochemical processes and physiological or phenotypic conditions in living organisms. However, to obtain unbiased data coverage of hundreds or thousands of metabolites remains a challenging task. Among the panel of available analytical methods, targeted and untargeted mass spectrometry approaches are among the most commonly used. While targeted metabolomics usually relies on multiple-reaction monitoring acquisition, untargeted metabolomics use either data-independent acquisition (DIA) or data-dependent acquisition (DDA) methods. Unlike DIA, DDA offers the possibility to get real, selective MS/MS spectra and thus to improve metabolite assignment when performing untargeted metabolomics. Yet, DDA settings are more complex to establish than DIA settings, and as a result, DDA is more prone to errors in method development and application. Here, we present a tutorial which provides guidelines on how to optimize the technical parameters essential for proper DDA experiments in metabolomics applications. This tutorial is organized as a series of rules describing the impact of the different parameters on data acquisition and data quality. It is primarily intended to metabolomics users and mass spectrometrists that wish to acquire both theoretical background and practical tips for developing effective DDA methods.
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