Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[α]pyrene

Polychaete worms can eliminate polycyclic aromatic hydrocarbons (PAHs) in environments through a mechanism that increases their water solubility. This detoxification starts with cytochrome P450 enzymes (CYPs) and then with glutathione S-transferases (GSTs). Here, a novel GST gene was identified and characterized from the widespread polychaete Perinereis aibuhitensis. The full-length cDNA of GST is 1544 bp and encodes 256 amino acids, belonging to the omega class. Gene expression patterns in P. aibuhitensis showed that its transcriptional level was positively correlated with the concentration of benzo[alpha]pyrene (0.5, 2, 4, and 8 mu g/L) exposure but was negatively correlated with a PAH benzene ring after it was exposed to the same mass concentrations of fluoranthene (3.2 mu g/L), phenanthrene (2.9 mu g/L), and benzo[alpha]pyrene (4.0 mu g/L) during the 14-day experimentation. These findings indicate that omega GST may play an important role in the phase II detoxification of PAHs in polychaete worms, and the persistence and bioavailability of PAHs may depend on benzene rings.

Automated summary

Polychaete worms can eliminate polycyclic aromatic hydrocarbons (PAHs) in environments by increasing their water solubility. A novel omega GST gene identified from Perinereis aibuhitensis plays an important role in the phase II detoxification of PAHs in polychaete worms. The gene expression of omega GST is positively correlated with the concentration of benzo[alpha]pyrene exposure but negatively correlated with the exposure of PAH benzene rings.