4.7 Article

Lipid-free tuna muscle samples are suitable for total mercury analysis

期刊

MARINE ENVIRONMENTAL RESEARCH
卷 169, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.marenvres.2021.105385

关键词

Methylmercury; Fat content; Delipidation; Yellowfin; Bigeye; Skipjack

资金

  1. MERTOX from the French Agence Nationale de la Recherche [ANR-17-CE34-0010]
  2. Agence Nationale de la Recherche (ANR) [ANR-17-CE34-0010] Funding Source: Agence Nationale de la Recherche (ANR)

向作者/读者索取更多资源

The study found that lipid extraction with dichloromethane did not affect mercury concentrations in tropical tuna, likely due to the affinity of methylmercury to proteins in tuna flesh, the low lipid content in tropical tuna muscle samples, and the non-polar nature of dichloromethane. This suggests that lipid-free samples commonly used in tropical tuna foraging ecology research can be equivalently used to document mercury concentrations at a global scale.
Tropical tunas are largely consumed worldwide, providing major nutritional benefits to humans, but also representing the main exposure to methylmercury, a potent neurotoxin that biomagnifies along food webs. The combination of ecological tracers (nitrogen and carbon stable isotopes, delta 15N and delta 13C) to mercury concentrations in tunas is scarce yet crucial to better characterize the influence of tuna foraging ecology on mercury exposure and bioaccumulation. Given the difficulties to get modern and historical tuna samples, analyses have to be done on available and unique samples. However, delta 13C values are often analysed on lipid-free samples to avoid bias related to lipid content. While lipid extraction with non-polar solvents is known to have no effect on delta 15N values, its impact on mercury concentrations is still unclear. We used white muscle tissues of three tropical tuna species to evaluate the efficiency and repeatability of different lipid extraction protocols commonly used in delta 13C and delta 15N analysis. Dichloromethane was more efficient than cyclohexane in extracting lipids in tuna muscle, while the automated method appeared more efficient but as repeatable as the manual method. Lipid extraction with dichloromethane had no effect on mercury concentrations. This may result from i) the affinity of methylmercury to proteins in tuna flesh, ii) the low lipid content in tropical tuna muscle samples, and iii) the non-polar nature of dichloromethane. Our study suggests that lipid-free samples, usually prepared for tropical tuna foraging ecology research, can be used equivalently to bulk samples to document in parallel mercury concentrations at a global scale.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据