Inhibitory effects of Prorocentrum donghaiense allelochemicals on Sargassum fusiformis zygotes probed by JIP-test based on fast chlorophyll fluorescence kinetics

The macro- and microalgae have been found to inhibit the growth and photosynthesis of one another due to allelopathic interactions between them. Sargassum fusiformis is a common and commercially cultivated seaweed in coastal waters of the East China Sea (ECS) and usually encounters dense harmful algal blooms (HABs) formed by dinoflagellates during their sexual reproduction period. In the present study, the effects of Prorocentrum donghaiense lipophilic extracted allelochemicals on the growth and photosynthesis of S. fusiformis zygotes were probed by fast chlorophyll fluorescence rise kinetics and chlorophyll a transient analysis (JIP-test). It was found that exposure to the allelochemicals led to decreased chlorophyll a content and photosynthetic rates of the zygotes in comparison to the ones in the control. In addition, using the JIP-test, it was found that the inhibitory effects of allelochemicals on photosynthesis of the zygotes were mainly exerted on the electron transport within PSII. The decrease of photosynthetic parameters such as V-J, Mo, phi(Po), phi(o), phi(Eo), PI, P-TR, P-ET in the zygotes exposed to the allelochemicals all revealed that the obstruction of electron transport, and the dominant decrease in P-ET, both implied that inhibition on the dark reaction contributed to the highest photosynthetic reduction. In addition, some reaction centers (RCs) in the zygotes exposed to the allelocamicals were inactivated, which led to higher dissipation of excitation energy, as demonstrated by the significant enhancement of the photosynthetic parameter DIo/RC. All the results indicated that the lipophilic extracts contained the allelochemicals of P. donghaiense which could inhibit the growth and photosynthesis of S. fusiformis zygotes by damaging the electron acceptors and inactivating RCs, and finally block the electron transport.

Automated summary

The lipophilic extracts from Prorocentrum donghaiense can inhibit the growth and photosynthesis of Sargassum fusiformis zygotes by damaging the electron acceptors and inactivating reaction centers, primarily blocking the electron transport during photosynthesis.