期刊
MARINE DRUGS
卷 19, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/md19080420
关键词
oyster; plasma; peptidome; antimicrobial peptides; cytotoxicity
资金
- National Science Foundation of China [32073002, 31902404]
- Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]
- Natural Science Foundation of Guangdong Province [2020A1515011533]
- China Agricultural Research System [CARS-49]
- Demonstration Project for Innovative Development of Marine Economy [NBHY-2017-S4]
- Zhejiang Provincial Top Key Discipline [KF2020009]
- Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]
- Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177]
Infection by Vibrio parahemolyticus significantly increased antimicrobial activity in oyster plasma, with URP20 showing promising antibacterial effects without cytotoxicity to mammalian cells. This study identifies novel bioactive peptides in marine mollusks and suggests further exploration for designing novel AMPs with broad applications.
Antimicrobial peptides are a fundamental component of mollusks' defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications.
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