期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 146, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.111393
关键词
Vine tea extract; 2R; 3R-Dihydromyricetin; Escherichia coli; Antibacterial activity; Membrane damage
资金
- National Natural Science Foundation of China, China [31401390]
The study investigated the antibacterial mechanisms of vine tea aqueous extract (VTE) and 2R, 3R-Dihydromyricetin (DMY) against Escherichia coli (E. coli), showing that they had good antibacterial activities by affecting cell membrane and nucleoid. The results suggest that VTE and DMY could be potential candidates for food preservatives due to their effective antibacterial activities and multiple targets.
In this study, the antibacterial mechanisms of vine tea aqueous extract (VTE) and 2R, 3R-Dihydromyricetin (DMY) against Escherichia coli (E. coli) were investigated. The results showed they had good antibacterial activities on E. coli, and affected cell membrane and nucleoid. The minimum bactericidal concentrations of VTE and DMY were 3.2 and 1.25 mg/mL, respectively. The constituent leakage tests showed VTE and DMY caused significant increases of extracellular alkaline phosphatase (24.4 +/- 0.4% and 5.3 +/- 0.1%) and beta-Galactosidase (27.6 +/- 1.0% and 13.6 +/- 0.5%), indicating the membrane integrity might be damaged. The lipids studies showed the ratio of unsaturated fatty acids to saturated fatty acids in treated groups were significant decrease (P < 0.05), indicating the membrane liquidity might be affected. Moreover, fluorescence experiments showed they could cause reduction of more than 82% of the DAPI-DNA and EB-DNA initial fluorescence intensity, indicating VTE and DMY could interacted with DNA through intercalate and grooving bindings. Furthermore, they showed good antibacterial activities in cabbage food model system. All over, the results suggested VTE and DMY might be applied as candidates of food preservatives for their good antibacterial activities and multiple targets.
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