期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 147, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.111605
关键词
Vitamin B3; NR; NMN; NAD plus; Saccharomyces cerevisiae; Hop; Beer
资金
- PRIN2017CBNCYT
This study quantified NR, NMN, and NAD+ in commercial craft beers and during fermentation, finding that adding hops significantly increased NR production. The findings serve as a baseline for further research on the interaction between yeast and hops.
In this study, nicotinamide riboside (NR), a form of vitamin B3, and its precursors nicotinamide mononucleotide (NMN) and NAD+ were quantitated by a fluorometric assay in ten commercial craft beers and throughout the fermentation process of a laboratory-scale beer. All tested beers from different commercial Saccharomyces cerevisiae strains contained NAD+ (ranging from 1.10 mu M to 17.80 mu M). NR concentrations ranged from 0.48 to 3.25 mu M, while the content of NMN was approximately 0.9 mu M. NR and NMN were only present in beers produced with S. cerevisiae strain US-05. Data from laboratory-scale beer productions using S. cerevisiae strain US-05 showed that the addition of hops [amarillo (alpha acid: 9.0%) (4 g/L) and centennial (alpha acid: 8.5%) (4 g/ L) varieties] at the 9th day of fermentation significantly increased NR production up to 6.11 mu M at the end of a typical fermentation of 21 days, and up to 11.42 mu M at 45 days of fermentation. The rapid increase in NR formation only occurred if both hops and yeast were present, and the burst was also confirmed in fermentations trials performed with S. cerevisiae strain CBS1171T and by replacing wort with YPD medium. This study can serve as baseline for further research on yeast-hop interaction.
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