期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 147, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.111504
关键词
Identification; Lactiplantibacillus; Lactobacillus; Intergenic spacer region; groESL
资金
- Ministry of Science and ICT of the Korean Government [NRF-2013M3A9A5076601]
- [KGM5492113]
Analysis of untranscribed DNA sequences in intergenic spacer regions (ISR) in groESL resolved ambiguity between closely related GRAS and non-GRAS species. Using groESL-ISR-specific primers for PCR sequencing accurately differentiated L. plantarum and L. pentosus, showing more accuracy than 16S rRNA sequences. Major probiotics species were grouped based on ISR sequences, which correlated with core-genome differentiation, indicating ISR-based species identification provides more accurate results than 16S rRNA-based identification and represents genome-based speciation.
The untranscribed DNA sequences of the intergenic spacer regions (ISR) in the groESL were analyzed to resolve the ambiguity within phylogenetically close GRAS and non-GRAS species. The sequencing results of amplified polymerase chain reaction (PCR) products using groESL-ISR-specific primers accurately distinguished L. plantarum and L. pentosus, which were not distinguished by 16 S rRNA sequences. Furthermore, the 20 selected major probiotics species were divided into several groups according to the length (22-91 bp) and homology (65-99%) of their ISR sequences, and this discrimination was consistent with core-genome-based differentiation. Therefore, ISR sequence-based species identification showed more accurate results than 16 S rRNA sequence-based identification and represented genome-based speciation.
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