期刊
LIFE SCIENCES
卷 274, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2021.119325
关键词
Luteolin; Quorum sensing; Pseudomonas aeruginosa; Virulence factors; Molecular docking
资金
- Fundamental Research Funds for the Central Universities [JUSRP11927]
- National Natural Science Foundation of China [51903108]
This study investigated the potential of luteolin as a quorum sensing inhibitor against Pseudomonas aeruginosa, demonstrating its effectiveness in inhibiting biofilm formation, virulence factor production, and bacterial motility. Luteolin was also found to reduce the accumulation of QS signaling molecules and downregulate the transcription of QS genes, suggesting its promising role in combating drug-resistant infections caused by P. aeruginosa.
Aims: The emergence of antibiotic tolerance was a tricky problem in the treatment of chronic Pseudomonas aeruginosa-infected cystic fibrosis and burn victims. The quorum sensing (QS) inhibitor may serve as a new tactic for the bacterial resistance by inhibiting the biofilm formation and the production of virulence factors. This study explored the potential of luteolin as a QS inhibitor against P. aeruginosa and the molecular mechanism involved. Main methods: Crystal violet staining, CLSM observation, and SEM analysis were carried out to assess the effect of luteolin on biofilm formation. The motility assays and the production of virulence factors were determined to evaluate the QS-inhibitory activity of luteolin. Acyl-homoserine lactone, RT-PCR, and molecular docking assays were conducted to explain its anti-QS mechanisms. Key findings: The biofilm formation, the production of virulence factors, and the motility of P. aeruginosa could be efficiently inhibited by luteolin. Luteolin could also attenuate the accumulation of the QS-signaling molecules N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) and N-butanoyl-L-homoserine lactone (BHL) (P < 0.01) and downregulate the transcription levels of QS genes (lasR, lasI, rhlR, and rhlI) (P < 0.01). Molecular docking analysis indicated that luteolin had a greater docking affinity with LasR regulator protein compared with OdDHL. Significance: This study is important as it reports the molecular mechanisms involved in the anti-biofilm formation activity of luteolin against P. aeruginosa. This study also indicated that luteolin could be helpful when used for the treatment of clinical drug-resistant infections of P. aeruginosa.
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