期刊
LIFE SCIENCES
卷 276, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2021.119405
关键词
miR-98; BCAT1; Cancer stem cells; Chemoresistance; Gastric cancer
资金
- CAMS Innovation Fund for Medical Sciences [2017-I2M-3-005]
- Independent Issue of State Key Laboratory of Molecular Oncology [SKL-2019-17]
This study demonstrates that miR-98 inhibits gastric cancer cell stemness and chemoresistance by targeting BCAT1, indicating that the miR-98/BCAT1 axis may serve as a potential therapeutic target in gastric cancer.
Aims: Gastric cancer stem cells (GCSCs) have been used as a therapeutic target. This study aims to estimate the role of miR-98-5p (termed miR-98) in the development of GCSCs. Main methods: The expression of miR-98 in CD44(+) GCSCs was verified by RT-PCR. The miR-98 was overexpressed in CD44(+) GCSCs by Lentivirus. The ability of self-renewal, invasion, chemoresistance and tumorigenicity was detected in vitro or in vivo after overexpression of miR-98. The target genes of miR-98 were predicted and verified by luciferase reporter assays. The effects miR-98/BCAT1 signaling on the chemoresistance and tumorigenicity of CD44(+) GCSCs were investigated in a xenograft model by rescue experiments. Key findings: We have shown that miR-98 was decreased in CD44(+) GCSCs. The overexpression of miR-98 could inhibit the expression of stem-related genes and the ability of self-renewal, invasion, and tumorigenicity of GCSCs. Also, we found that miR-98 overexpression enhances the sensitivity to cisplatin treatment in vitro. Using a xenograft model, we showed that miR-98 overexpression reversed paclitaxel resistance to CD44(+) GCSCs. Finally, we found that branched-chain aminotransferases 1 (BCAT1) is a target gene of miR-98. Overexpressed BCAT1 reversed xenograft tumor formation ability and attenuated the paclitaxel chemosensitivity induced by miR-98 downregulation. Furthermore, BCAT1 restoration affected the expression of invasion and drug resistance-related genes. Significance: This study revealed miR-98 inhibits gastric cancer cell stemness and chemoresistance by targeting BCAT1, suggesting that this miR-98/BCAT1 axis represents a potential therapeutic target in gastric cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据